Adams, P. D., Arkin, I. T., Engelman, D. M. and BrŸnger, A. T. (1995) Computational searching and mutagenesis suggest a structure for the pentameric transmembrane domain of phospholamban. Nature Struct Biol 2, 154-162.

Allen, J., Feher, G., Yeates, T., Komiya, H. and Rees, D. (1997) Structure of the reaction center from Rhodobacter sphaeroides R-26: The protein subunits. Proc Natl Acad Sci USA 84, 6162-6166.

Altenbach, C., Flitsch, S., Khorana, H. and Hubbell, W. (2001) Structural studies in transmembrane proteins. 2. Spin labeling of bacteriorhodopsin mutants at unique cysteines. Biochemistry 28, 7806-7812.

Altenbach, C., Greenhalg, D. A., Khorana, H. G. and Hubbell, W. L. (1994) A collision gradient method to determine the immersion depth of nitroxides in lipid bilayers: Application to spin-labeled mutants of bacteriorhodopsin. Proc Natl Acad Sci USA 91, 1667-1671.

Altenbach, C., Marti, T., Khorana, H. G. and Hubbell, W. L. (1990) Transmembrane Protein Structure - Spin Labeling of Bacteriorhodopsin Mutants. Science 248, 1088-1092.

Andersson, H. and von Heijne, G. (1993) Sec-dependent and sec-independent assembly of E. coli inner membrane proteins - the topological rules depend on chain length. EMBO J 12, 683-691.

Andersson, H. and von Heijne, G. (1994a) Membrane protein topology: Effects of DmH+ on the translocation of charged residues explain the ‘positive inside’ rule. EMBO J 13, 2267-2272.

Andersson, H. and von Heijne, G. (1994b) Positively charged residues influence the degree of sec-dependence in protein translocation across the E. coli inner membrane. FEBS Lett 347, 169-172.

Arkin, I. T., Adams, P. D., Mackenzie, K. R., Lemmon, M. A., BrŸnger, A. T. and Engelman, D. M. (1994) Structural organization of the pentameric transmembrane a-helices of phospholamban, a cardiac ion channel. EMBO J 13, 4757-4764.

Arkin, I. T., MacKenzie, K. R., Fisher, L., Aimoto, S., Engelman, D. M. and Smith, S. O. (1996) Mapping the lipid-exposed surfaces of membrane proteins. Nature Struct Biol 3, 240-243.

Arkin, I. T., Rothman, M., Ludlam, C. F. C., Aimoto, S., Engelman, D. M., Rothschild, K. J. and Smith, S. O. (1995) Structural model of the phospholamban ion channel complex in phospholipid membranes. J Mol Biol 248, 824-834.

Bacher, G., Lutcke, H., Jungnickel, B., Rapoport, T. A. and Dobberstein, B. (1996) Regulation by the ribosome of the GTPase of the signal- recognition particle during protein targeting. Nature 381, 248-251.

Baldwin, J. M. (1993) The probable arrangement of the helices in G protein-coupled receptors. EMBO J 12, 1693-1703.

Bechinger, B., Zasloff, M. and Opella, S. J. (1993) Structure and Orientation of the Antibiotic Peptide Magainin in Membranes by Solid-State Nuclear Magnetic Resonance Spectroscopy. Protein Sci 2, 2077-2084.

Berghšfer, J., Karnauchov, I., Herrmann, R. G. and Klšsgen, R. B. (1995) Isolation and characterization of a cDNA encoding the SecA protein from spinach chloroplasts - Evidence for azide resistance of Sec-dependent protein translocation across thylakoid membranes in spinach. J Biol Chem 270, 18341-18346.

Borel, A. C. and Simon, S. M. (1996) Biogenesis of polytopic membrane proteins: membrane segments assemble with translocation channels prior to membrane integration. Cell 85, 379-389.

Bosch, D., Leunissen, J., Verbakel, J., de Jong, M., van Erp, H. and Tommassen, J. (1996) Periplasmic accumulation of truncated forms of outer-membrane PhoE protein of Escherichia coli K-12. J Mol Biol 189, 449-55.

Boyd, D., Traxler, B. and Beckwith, J. (1993) Analysis of the Topology of a Membrane Protein by Using a Minimum Number of Alkaline Phosphatase Fusions. J Bacteriol 175, 553-556.

Braun, P., Persson, B., Kaback, H. R. and von Heijne, G. (1996) Alanine insertion scanning mutagenesis of lactose permease transmembrane helices. Identification of a structurally and functionally important segment in helix III. submitted.

Cao, G. Q. and Dalbey, R. E. (1994) Translocation of N-terminal tails across the plasma membrane. EMBO J 13, 4662-4669.

Cao, G. Q., Kuhn, A. and Dalbey, R. E. (1995) The translocation of negatively charged residues across the membrane is driven by the electrochemical potential: Evidence for an electrophoresis-like membrane transfer mechanism. EMBO J 14, 866-875.

Chen, H. F. and Kendall, D. A. (1995) Artificial transmembrane segments - Requirements for stop transfer and polypeptide orientation. J Biol Chem 270, 14115-14122.

Chen, R. and Henning, U. (1996) A periplasmic protein (Skp) of Escherichia coli selectively binds a class of outer membrane proteins. Mol Microbiol 19, 1287-1294.

Claros, M. G. and von Heijne, G. (1994) TopPred II: An improved software for membrane protein structure prediction. CABIOS 10, 685-686.

Connolly, T. and Gilmore, R. (1993) GTP Hydrolysis by Complexes of the Signal Recognition Particle and the Signal Recognition Particle Receptor. J Cell Biol 123, 799-807.

Contifine, B. M., Lei, S. J. and McLane, K. E. (1996) Antibodies as tools to study the structure of membrane proteins: The case of the nicotinic acetylcholine receptor. Annu Rev Biophys Biomol Struc 25, 197-229.

Cornette, J. L., Cease, K. B., Margalit, H., Spouge, J. L., Berzofsky, J. A. and De Lisi, C. (1997) Hydrophobicity scales and computational techniques for detecting amphipathic structures in proteins. J Mol Biol 195, 659-685.

Cowan, S. W. and Rosenbusch, J. P. (1994) Folding pattern diversity of integral membrane proteins. Science 264, 914-916.

Cowan, S. W., Schirmer, T., Rummel, G., Steiert, M., Ghosh, R., Pauptit, R. A., Joansonius, J. N. and Rosenbusch, J. P. (1992) Crystal structures explain functional properties of two E. coli porins. Nature 358, 727-733.

Cserzo, M., Bernassau, J. M., Simon, I. and Maigret, B. (1994) New alignment strategy for transmembrane proteins. J Mol Biol 243, 388-396.

Dalbey, R. E. and von Heijne, G. (1992) Signal Peptidases in Prokaryotes and Eukaryotes - A New Protease Family. Trends Biochem Sci 17, 474-478.

De Cock, H., Van Blokland, S. and Tommassen, J. (1996) In vitro insertion and assembly of outer membrane protein PhoE of Escherichia coli K-12 into the outer membrane - Role of Triton X-100. J Biol Chem 271, 12885-12890.

Deisenhofer, J., Epp, O., Miki, K., Huber, R. and Michel, H. (1995) Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3� resolution. Nature 318, 618-624.

Dekker, N., Merck, K., Tommassen, J. and Verheij, H. M. (1995) In vitro folding of Escherichia coli outer-membrane phospholipase A. Eur J Biochem 232, 214-219.

Diamond, D. L., Strobel, S., Chun, S. Y. and Randall, L. L. (1995) Interaction of SecB with intermediates along the folding pathway of maltose-binding protein. Protein Sci 4, 1118-1123.

Do, H., Falcone, D., Lin, J., Andrews, D. W. and Johnson, A. E. (1996) The cotransational integration of membrane proteins into the phospholipid bilayer is multistep process. Cell 85, 369-378.

Douglas, S. E. (1992) A secY homologue is found in the plastid genome of Cryptomonas F. FEBS Lett 299, 93-96.

Dunten, R. L., Sahintoth, M. and Kaback, H. R. (1993) Role of the Charge Pair Aspartic Acid-237-Lysine-358 in the Lactose Permease of Escherichia coli. Biochemistry 32, 3139-3145.

Economou, A., Pogliano, J. A., Beckwith, J., Oliver, D. B. and Wickner, W. (1995) SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF. Cell 83, 1171-1181.

Economou, A. and Wickner, W. (1994) SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion. Cell 78, 835-843.

Efremov, R. G. and Vergoten, G. (1996) Hydrophobic organization of alpha-helix membrane bundle in bacteriorhodopsin. J Protein Chem 15, 63-76.

Ehrmann, M., Boyd, D. and Beckwith, J. (1990) Genetic Analysis of Membrane Protein Topology by a Sandwich Gene Fusion Approach. Proc Natl Acad Sci USA 87, 7574-7578.

Elofsson, A., Wallin, E., Tsukihara, T., Yoshikawa, S. and von Heijne, G. (1996) Anatomy of helix bundle membrane proteins. Submitted.

Engelman, D. M. and Steitz, T. A. (1991) The spontaneous insertion of proteins into and across membranes: The helical hairpin hypothesis. Cell 23, 411-422.

Gafvelin, G., Sakaguchi, M., Andersson, H. and von Heijne, G. (1996) Topological rules for membrane protein assembly in eukaryotic cells. Submitted.

Gafvelin, G. and von Heijne, G. (1994) Topological "frustration" in multi-spanning E. coli inner membrane proteins. Cell 77, 401-412.

GŠrtner, F., Bšmer, U., Guiard, B. and Pfanner, N. (1995) The sorting signal of cytochrome b(2) promotes early divergence from the general mitochondrial import pathway and restricts the unfoldase activity of matrix Hsp70. EMBO J 14, 6043-6057.

Gavel, Y., Steppuhn, J., Herrmann, R. and von Heijne, G. (1991) The Positive-Inside Rule Applies to Thylakoid Membrane Proteins. FEBS Lett 282, 41-46.

Gavel, Y. and von Heijne, G. (1992) The Distribution of Charged Amino Acids in Mitochondrial Inner Membrane Proteins Suggests Different Modes of Membrane Integration for Nuclearly and Mitochondrially Encoded Proteins. Eur J Biochem 205, 1207-1215.

Gebert, J. F., Overhoff, B., Manson, M. and Boos, W. (1998) The Tsr Chemosensory Transducer of Escherichia coli Assembles into the Cytoplasmic Membrane via a SecA-dependent Process. J Biol Chem 263, 16652-16660.

Gelman, M. S., Chang, W. S., Thomas, D. Y., Bergeron, J. J. M. and Prives, J. M. (1995) Role of the endoplasmic reticulum chaperone calnexin in subunit folding and assembly of nicotinic acetylcholine receptors. J Biol Chem 270, 15085-15092.

Glick, B. S., Brandt, A., Cunningham, K., Muller, S., Hallberg, R. L. and Schatz, G. (1992) Cytochromes-c1 and Cytochromes-b2 Are Sorted to the Intermembrane Space of Yeast Mitochondria by a Stop-Transfer Mechanism. Cell 69, 809-822.

Grigorieff, N., Ceska, T. A., Downing, K. H., Baldwin, J. M. and Henderson, R. (1996) Electron-crystallographic refinement of the structure of bacteriorhodopsin. J Mol Biol 259, 393-421.

Grisshammer, R. and Tate, C. G. (1995) Overexpression of integral membrane proteins for structural studies. Quart Rev Biophys 28, 315-422.

Hardy, S. J. S. and Randall, L. L. (1991) A kinetic partitioning model of selective binding of nonnative proteins by the bacterial chaperone SecB. Science 251, 439-443.

Hartl, F. U., Lecker, S., Schiebel, E., Hendrick, J. P. and Wickner, W. (1990) The Binding Cascade of SecB to SecA to SecY/E Mediates Preprotein Targeting to the E. Coli Plasma Membrane. Cell 63, 269-279.

Hartl, F. U. and Neupert, W. (1990) Protein Sorting to Mitochondria - Evolutionary Conservations of Folding and Assembly. Science 247, 930-938.

He, M. M., Voss, J., Hubbell, W. L. and Kaback, H. R. (1995a) Use of designed metal-binding sites to study helix proximity in the lactose permease of Escherichia coli .1. Proximity of helix VII (Asp237 and Asp240) with helices X (Lys319) and XI (Lys358). Biochemistry 34, 15661-15666.

He, M. M., Voss, J., Hubbell, W. L. and Kaback, H. R. (1995b) Use of designed metal-binding sites to study helix proximity in the lactose permease of Escherichia coli .2. Proximity of helix IX (Arg302) with helix X (His322 and Glu325). Biochemistry 34, 15667-15670.

Helenius, A. (1994) How N-Linked oligosaccharides affect glycoprotein folding in the endoplasmic reticulum. Mol Biol Cell 5, 253-265.

Henderson, R., Baldwin, J. M., Ceska, T. A., Zemlin, F., Beckmann, E. and Downing, K. H. (1990) A Model for the Structure of Bacteriorhodopsin Based on High Resolution Electron Cryo-Microscopy. J Mol Biol 213, 899-929.

Henderson, R. and Unwin, P. N. T. (1975) Three-dimensional model of purple membrane obtained by electron microscopy. Nature 257, 28-32.

Hennessey, E. S. and Broome-Smith, J. K. (1993) Gene-Fusion Techniques for Determining Membrane-Protein Topology. Curr Opin Struct Biol 3, 524-531.

Hennessey, E. S., Hashemzadeh-Bonehi, L., Hunt, L. A. and Broome-Smith, J. K. (1993) Assembly of eukaryotic class-III (N-out, C-in) membrane proteins into the Escherichia coli cytoplasmic membrane. FEBS Lett 331, 159-161.

Herrmann, J. M., Koll, H., Cook, R. A., Neupert, W. and Stuart, R. A. (1995) Topogenesis of cytochrome oxidase subunit II - Mechanisms of protein export from the mitochondrial matrix. J Biol Chem 270, 27079-27086.

Herzyk, P. and Hubbard, R. E. (1995) Automated method for modeling seven-helix transmembrane receptors from experimental data. Biophys J 69, 2419-2442.

High, S. (1995) Protein translocation at the membrane of the endoplasmic reticulum. Prog Biophys Mol Biol 63, 233-250.

Ito, K. (1995) Protein translocation genetics. In Membrane Organization and Biogenesis in Prokaryotic Organisms, (ed. R. D. Dalbey), JAI Press.

Iwata, S., Ostermeier, C., Ludwig, B. and Michel, H. (1995) Structure at 2.8 � resolution of cytochrome c oxidase from Paracoccus denitrificans. Nature 376, 660-669.

Jennings, M. L. (2001) Topography of membrane proteins. Annu Rev Biochem 58, 999-1027.

Jones, D. T., Taylor, W. R. and Thornton, J. M. (1994) A model recognition approach to the prediction of all-helical membrane protein structure and topology. Biochemistry 33, 3038-3049.

Jung, K., Jung, H., Wu, J., Prive, G. G. and Kaback, H. R. (1993) Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli. Biochemistry 32, 12273-12278.

Jung, K., Voss, J., He, M., Hubbell, W. L. and Kaback, H. R. (1995) Engineering a metal binding site within a polytopic membrane protein, the lactose permease of Escherichia coli. Biochemistry 34, 6272-6277.

Jungnickel, B., Rapoport, T. A. and Hartmann, E. (1994) Protein translocation: Common themes from bacteria to man. FEBS Lett 346, 73-77.

Kaback, H. R. (1996) The lactose permease of Escherichia coli: Past, present, and future. In Handbook of biological physics: Transport processes in eukaryotic and prokaryotic organisms, (eds. W. N. Konings and H. R. Kaback), pp. in press, Elsevier, Amsterdam.

Kaback, H. R., Frillingos, S., Jung, H., Jung, K., Prive, G. G., Ujwal, M. L., Weitzman, C., Wu, J. and Zen, K. (1994) The lactose permease meets Frankenstein. J Exp Biol 196, 183-195.

Kelleher, D. J., Kreibich, G. and Gilmore, R. (1992) Oligosaccharyltransferase Activity is Associated with a Protein Complex Composed of Robophorins I and II and a 48 kd Protein. Cell 69, 55-65.

Klose, M., Schwarz, H., MacIntyre, S., Freudl, R., Eschbach, M. L. and Henning, U. (1998) Internal deletions in the gene for an Escherichia coli outer membrane protein define an area possibly important for recognition of the outer membrane by this polypeptide. J Biol Chem 263, 13291-6.

Klose, M., Storiko, A., Stierhof, Y. D., Hindennach, I., Mutschler, B. and Henning, U. (1993) Membrane Assembly of the Outer Membrane Protein OmpA of Escherichia-coli. J Biol Chem 268, 25664-25670.

Kraulis, P. J. (1991) MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures. J Appl Crystallogr 24, 946-950.

Kreusch, A., Neubuser, A., Schiltz, E., Weckesser, J. and Schulz, G. E. (1994) Structure of the Membrane Channel Porin from Rhodopseudomonas blastica at 2.0 � Resolution. Protein Sci 3, 58-63.

KŸhlbrandt, W. (1992) Two-Dimensional Crystallization of Membrane Proteins. Quart Rev Biophys 25, 1-49.

KŸhlbrandt, W. and Wang, D. N. (1991) Three-dimensional structure of plant light-harvesting complex determined by electron crystallography. Nature 350, 130-134.

KŸhlbrandt, W., Wang, D. N. and Fujiyoshi, Y. (1994) Atomic model of plant light-harvesting complex by electron crystallography. Nature 367, 614-621.

Kuroiwa, T., Sakaguchi, M., Mihara, K. and Omura, T. (1991) Systematic Analysis of Stop-Transfer Sequence for Microsomal Membrane. J Biol Chem 266, 9251-9255.

Kutay, U., Ahnerthilger, G., Hartmann, E., Wiedenmann, B. and Rapoport, T. A. (1995) Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane. EMBO J 14, 217-223.

Kyte, J. and Doolittle, R. F. (1992) A Simple Method for Displaying the Hydropathic Character of a Protein. J Mol Biol 157, 105-132.

Laidler, V., Chaddock, A. M., Knott, T. G., Walker, D. and Robinson, C. (1995) A SecY homolog in Arabidopsis thaliana - Sequence of a full-length cDNA clone and import of the precursor protein into chloroplasts. J Biol Chem 270, 17664-17667.

Lakey, J. H., Duche, D., Gonzalezmanas, J. M., Baty, D. and Pattus, F. (1993) Fluorescence Energy Transfer Distance Measurements - The Hydrophobic Helical Hairpin of Colicin-A in the Membrane Bound State. J Mol Biol 230, 1055-1067.

Lakey, J. H., Parker, M. W., Gonzalezmanas, J. M., Duche, D., Vriend, G., Baty, D. and Pattus, F. (1994) The Role of Electrostatic Charge in the Membrane Insertion of Colicin a - Calculation and Mutation. Eur J Biochem 220, 155-163.

Lanyi, J. K. (1995) Bacteriorhodopsin as a model for proton pumps. Nature 375, 461-462.

Lazar, S. W. and Kolter, R. (1996) SurA assists the folding of Escherichia coli outer membrane proteins. J Bacteriol 178, 1770-1773.

Lee, G. F., Burrows, G. G., Lebert, M. R., Dutton, D. P. and Hazelbauer, G. L. (1994) Deducing the organization of a transmembrane domain by disulfide crosslinking. The bacterial chemoreceptor Trg. J Biol Chem 269, 29920-29927.

Lee, G. F. and Hazelbauer, G. L. (1995) Quantitative approaches to utilizing mutational analysis and disulfide crosslinking for modeling a transmembrane domain. Prot Science 4, 1100-1107.

Lee, J.-I., Hwang, P. P., Hansen, C. and Wilson, T. H. (1992a) Possible salt bridges between transmembrane a-helices of the lactose carrier of Escherichia coli. J Biol Chem 267, 20758-20764.

Lee, J.-I., Hwang, P. P. and Wilson, T. H. (1993) Lysine 319 interacts with both glutamic acid 269 and aspartic acid 240 in the lactose carrier of Escherichia coli. J Biol Chem 268, 20017-20015.

Lee, J. I., Kuhn, A. and Dalbey, R. E. (1992b) Distinct Domains of an Oligotopic Membrane Protein Are Sec-Dependent and Sec-Independent for Membrane Insertion. J Biol Chem 267, 938-943.

Lemmon, M., Flanagan, J., Hunt, J., Adair, B., Bormann, B.-J., Dempsey, C. and Engelman, D. (1992a) Glycophorin A dimerization is driven by specific interactions between transmembrane a-helices. J Biol Chem 267, 7683-7689.

Lemmon, M. A. and Engelman, D. M. (1994) Specificity and promiscuity in membrane helix interactions. FEBS Lett 346, 17-20.

Lemmon, M. A., Flanagan, J. M., Treutlein, H. R., Zhang, J. and Engelman, D. M. (1992b) Sequence specificity in the dimerization of transmembrane a-helices. Biochemistry 31, 12719-12725.

Lemmon, M. A., Treutlein, H. R., Adams, P. D., BrŸnger, A. T. and Engelman, D. M. (1994) A dimerization motif for transmembrane a-helices. Nature Struct Biol 1, 157-163.

Lipp, J., Flint, N., Haeuptle, M.-T. and Dobberstein, B. (2001) Structural requirements for membrane assembly of proteins spanning the membrane several times. J Cell Biol 109, 2013-2022.

Ludlam, C. F. C., Arkin, I. T., Liu, X. M., Rothman, M. S., Rath, P., Aimoto, S., Smith, S. O., Engelman, D. M. and Rothschild, K. J. (1996) Fourier transform infrared spectroscopy and site-directed isotope labeling as a probe of local secondary structure in the transmembrane domain of phospholamban. Biophys J 70, 1728-1736.

Luirink, J. and Dobberstein, B. (1994) Mammalian and Escherichia coli signal recognition particles. Mol Microbiol 11, 9-13.

MacIntyre, S., Freudl, R., Eschbach, M.-L. and Henning, U. (1998) An artificial hydrophobic sequence functions as either an anchor or a signal sequence at only one of two positions within the Escherichia coli outer membrane protein OmpA. J.Biol.Chem. 263, 19053-59.

Manoil, C. (1991) Analysis of membrane protein topology using alkaline phosphatase and §-galactosidase gene fusions. Methods in Cell Biol 34, 61-75.

Manoil, C. and Traxler, B. (1995) Membrane protein assembly: Genetic, evolutionary and medical perspectives. Annu Rev Genet 29, 131-150.

Mant, A., Skovgaard-Nielsen, V., Knott, T. G., Lindberg-M¿ller, B. and Robinson, C. (1994) Multiple mechanisms for the targeting of photosystem I subunits F, H, K, L, and N into and across the thylakoid membrane. J Biol Chem 269, 27303-27309.

McDermott, G., Price, S. M., Freer, A. A., Hawthornthwaite-Lawless, A. M., Papiz, M. Z., Cogdell, R. J. and Isaacs, N. W. (1995) Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature 374, 517-521.

Miller, J. D., Wilhelm, H., Gierasch, L., Gilmore, R. and Walter, P. (1993) GTP Binding and Hydrolysis by the Signal Recognition Particle During Initiation of Protein Translocation. Nature 366, 351-354.

Mingarro, I., Whitley, P., Lemmon, M. A. and von Heijne, G. (1996) Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix. A rapid way to map helix-helix interactions in integral membrane proteins. Protein Sci 5, 1339-1341.

Miroux, B. and Walker, J. E. (1996) Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol 260, 289-299.

Nakai, K. and Kanehisa, M. (1991) Expert System for Predicting Protein Localization Sites in Gram-Negative Bacteria. Proteins: Struct Funct Genet 11, 95-110.

Nakai, M., Goto, A., Nohara, T., Sugita, D. and Endo, T. (1994) Identification of the SecA protein homolog in pea chloroplasts and its possible involvement in thylakoidal protein transport. J Biol Chem 269, 31338-31341.

Nakai, M., Sugita, D., Omata, T. and Endo, T. (1993) Sec-Y Protein Is Localized in Both the Cytoplasmic and Thylakoid Membranes in the Cyanobacterium Synechococcus PCC7942. Biochem Biophys Res Commun 193, 228-234.

Nilsson, I. and von Heijne, G. (1993) Determination of the Distance Between the Oligosaccharyltransferase Active Site and the Endoplasmic Reticulum Membrane. J Biol Chem 268, 5799-5801.

Nilsson, I., Whitley, P. and von Heijne, G. (1994) The C-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase. J Cell Biol 126, 1127-1132.

Nishiyama, K., Suzuki, T. and Tokuda, H. (1996) Inversion of the Membrane Topology of SecG Coupled with SecA-Dependent Preprotein Translocation. Cell 85, 71-81.

Oliver, D. B., Cabelli, R. J., Dolan, K. M. and Jarosik, G. P. (1990) Azide-Resistant Mutants of Escherichia coli Alter the SecA-Protein, an Azide-Sensitive Component of the Protein Export Machinery. Proc Natl Acad Sci USA 87, 8227-8231.

Oliver, J., Jungnickel, B., Gšrlich, D., Rapoport, T. and High, S. (1995) The sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum. FEBS Lett 362, 126-130.

Ostermann, J., Horwich, A. L., Neupert, W. and Hartl, F. U. (2001) Protein Folding in Mitochondria Requires Complex Formation with Hsp60 and ATP Hydrolysis. Nature 341, 125-130.

Pakula, A. A. and Simon, M. I. (1992) Determination of Transmembrane Protein Structure by Disulfide Cross-Linking - The Escherichia coli Tar Receptor. Proc Natl Acad Sci USA 89, 4144-4148.

Parker, M., Pattus, F., Tucker, A. and Tsernoglou, D. (2001) Structure of the membrane-pore-forming fragment of colicin A. Nature 337, 93-96.

Parker, M. W., Buckley, J. T., Postma, J. P. M., Tucker, A. D., Leonard, K., Pattus, F. and Tsernoglou, D. (1994) Structure of the Aeromonas Toxin Proaerolysin in Its Water-Soluble and Membrane-Channel States. Nature 367, 292-295.

Parker, M. W. and Pattus, F. (1993) Rendering a Membrane Protein Soluble in Water - A Common Packing Motif in Bacterial Protein Toxins. Trends Biochem Sci 18, 391-395.

Persson, B. and Argos, P. (1996) Topology prediction of membrane proteins. Protein Sci 5, 363-371.

Peterson, J. R., Ora, A., Van, P. N. and Helenius, A. (1995) Transient, lectin-like association of calreticulin with folding intermediates of cellular and viral glycoproteins. Mol Biol Cell 6, 1173-1184.

Picot, D. and Garavito, R. M. (1994) Prostaglandin H synthase: Implications for membrane structure. FEBS Lett 346, 21-25.

Picot, D., Loll, P. J. and Garavito, R. M. (1994) The X-Ray Crystal Structure of the Membrane Protein Prostaglandin-H(2) Synthase-1. Nature 367, 243-249.

Pind, S., Riordan, J. R. and Williams, D. B. (1994) Participation of the endoplasmic reticulum chaperone calnexin (p88, IP90) in the biogenesis of the cystic fibrosis transmembrane conductance regulator. J Biol Chem 269, 12784-12788.

Popot, J.-L. and de Vitry, C. (1990) On the microassembly of integral membrane proteins. Annu.Rev.Biophys.Biophys.Chem. 19, 369-403.

Popot, J.-L. and Saraste, M. (1995) Engineering membrane proteins. Curr Opin Biotech 6, 394-402.

Popot, J. L. and Engelman, D. M. (1990) Membrane Protein Folding and Oligomerization - The 2-Stage Model. Biochemistry 29, 4031-4037.

Prinz, W. A. and Beckwith, J. (1994) Gene fusion analysis of membrane protein topology: A direct comparison of alkaline phosphatase and §-lactamase fusions. J Bacteriol 176, 6410-6413.

Randall, L. L. (1992) Peptide Binding by Chaperone SecB - Implications for Recognition of Nonnative Structure. Science 257, 241-245.

Rapiejko, P. J. and Gilmore, R. (1994) Signal sequence recognition and targeting of ribosomes to the endoplasmic reticulum by the signal recognition particle do not require GTP. Mol Biol Cell 5, 887-897.

Rapoport, T. A. (1992) Transport of Proteins Across the Endoplasmic Reticulum Membrane. Science 258, 931-936.

Rees, D. C., DeAntonio, L. and Eisenberg, D. (2001a) Hydrophobic Organization of Membrane Proteins. Science 245, 510-13.

Rees, D. C., Komiya, H., Yeates, T. O., Allen, J. P. and Feher, G. (2001b) The bacterial photosynthetic reaction center as a model for membrane proteins. Annu Rev Biochem 58, 607-33.

Reithmeier, R. A. F. (1995) Characterization and modelling of membrane proteins using sequence analysis. Curr Opinon Struct Biol 5, 491-500.

Rojo, E. E., Stuart, R. A. and Neupert, W. (1995) Conservative sorting of F0-ATPase subunit 9: Export from matrix requires Delta pH across inner membrane and matrix ATP. EMBO J 14, 3445-3451.

Rospert, S., MŸller, S., Schatz, G. and Glick, B. S. (1994) Fusion proteins containing the cytochrome b2 presequence are sorted to the mitochonrial intermembrane space independently of hsp60. J Biol Chem 269, 17279-17288.

Rost, B., Casadio, R., Fariselli, P. and Sander, C. (1995) Transmembrane helices predicted at 95% accuracy. Protein Sci 4, 521-533.

Rost, B., Fariselli, P. and Casadio, R. (1996) Topology prediction for helical transmembrane segments at 86% accuracy. manuscript.

Rothman, J. E. (1996) The protein machinery of vesicle budding and fusion. Protein Sci 5, 185-194.

Sahin-Toth, M., Dunten, R. L. and Kaback, H. R. (1995) Design of a membrane protein for site-specific proteolysis: Properties of engineered factor Xa protease sites in the lactose permease of Escherichia coli. Biochemistry 34, 1107-1112.

Samatey, F. A., Xu, C. and Popot, J.-C. (1995) On the distribution of amino acid residues in transmembrane alpha-helix bundles. Proc Natl Acad Sci USA 92, 4577-4581.

Scaramuzzi, C. D., Hiller, R. G. and Stokes, H. W. (1992a) Identification of a Chloroplast-Encoded secA Gene Homologue in a Chromophytic Alga - Possible Role in Chloroplast Protein Translocation. Curr Genetics 22, 421-427.

Scaramuzzi, C. D., Stokes, H. W. and Hiller, R. G. (1992b) Characterisation of a Chloroplast-Encoded sec-Y Homologue and atpH from a Chromophytic Alga - Evidence for a Novel Chloroplast Genome Organisation. FEBS Lett 304, 119-123.

Schatz, G. and Dobberstein, B. (1996) Common principles of protein translocation across membranes. Science 271, 1519-1526.

Schertler, G. F. X., Villa, C. and Henderson, R. (1993) Projection structure of rhodopsin. Nature 362, 770-772.

Schirmer, T. and Cowan, S. W. (1993) Prediction of membrane-spanning §-strands and its application to maltoporin. Prot Sci 2, 1361-1363.

Schirmer, T., Keller, T. A., Wang, Y. F. and Rosenbusch, J. P. (1995) Structural basis for sugar translocation through maltoporin channels at 3.1 angstrom resolution. Science 267, 512-514.

Schwartz, T. W., Gether, U., Schambye, H. T. and Hjort, S. A. (1995) Molecular mechanism of action of non-peptide ligands for peptide receptors. Curr Pharmaceut Design 1, 325-342.

Silberstein, S., Kelleher, D. and Gilmore, R. (1992) The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1. J Biol Chem 267, 23658-23663.

Simmerman, H. K. B., Kobayashi, Y. M., Autry, J. M. and Jones, L. R. (1996) A leucine zipper stabilizes the pentameric membrane domain of phospholamban and forms a coiled-coil pore structure. J Biol Chem 271, 5941-5946.

Spiess, M. (1995) Heads or tails - What determines the orientation of proteins in the membrane. FEBS Lett 369, 76-79.

Surrey, T. and JŠhnig, F. (1992) Refolding and Oriented Insertion of a Membrane Protein into a Lipid Bilayer. Proc Natl Acad Sci USA 89, 7457-7461.

Surrey, T., Schmid, A. and Jahnig, F. (1996) Folding and membrane insertion of the trimeric beta-barrel protein OmpF. Biochemistry 35, 2283-2288.

Suwa, M., Hirokawa, T. and Mitaku, S. (1995) A continuum theory for the prediction of lateral and rotational positioning of alpha-helices in membrane proteins: Bacteriorhodopsin. Protein-Struct Funct Genet 22, 363-377.

SŠŠf, A., Andersson, H., Gafvelin, G. and von Heijne, G. (1995) SecA-dependence of the translocation of a large periplasmic loop in the E. coli MalF inner membrane protein is a function of sequence context. Mol Membr Biol 12, 209-215.

SŠŠf, A., Wallin, E. and von Heijne, G. (1996) Translocation of marginally hydrophobic segments across prokarytoic and eukaryotic membranes. Submitted.

Taylor, W. R., Jones, D. T. and Green, N. M. (1994) A Method for a-Helical Integral Membrane Protein Fold Prediction. Proteins - Struct Funct Genet 18, 281-294.

Traxler, B. and Murphy, C. (1996) Insertion of the polytopic membrane protein MalF is dependent on the bacterial secretion machinery. J Biol Chem 271, 12394-12400.

Treutlein, H. R., Lemmon, M. A., Engelman, D. M. and BrŸnger, A. T. (1992) The glycophorin A transmembrane domain dimer: sequence-specific propensity for a right-handed supercoil of helices. Biochemistry 31, 12726-12732.

Tsukihara, T., Aoyama, H., Yamashita, E., Tomizaki, T., Yamaguchi, H., Shinzawa-Itoh, K., Nakashima, R., Yaono, R. and Yoshikawa, S. (1995) Structures of metal sites of oxidized bovine heart cytochrome c oxidase at 2.8 �. Science 269, 1069-1064.

Tsukihara, T., Aoyama, H., Yamashita, E., Tomizaki, T., Yamaguchi, H., Shinzawa-Itoh, K., Nakashima, R., Yaono, R. and Yoshikawa, S. (1996) The whole stucture of the 13-subunit oxidized cytochrome c oxidase at 2.8 �. Science 272, 1136-1144.

Tucker, J. and Grisshammer, R. (1996) Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem J in press.

Tuffery, P., Etchebest, C., Popot, J. L. and Lavery, R. (1994) Prediction of the Positioning of the Seven Transmembrane a-Helices of Bacteriorhodopsin - A Molecular Simulation Study. J Mol Biol 236, 1105-1122.

Uchida, K., Mori, H. and Mizushima, S. (1995) Stepwise movement of preproteins in the process of translocation across the cytoplasmic membrane of Escherichia coli. J Biol Chem 270, 30862-30868.

van der Goot, F. G., Gonzalez-Manas, J. M., Lakey, J. H. and Pattus, F. (1991) A Molten-Globule Membrane-Insertion Intermediate of the Pore-Forming Domain of Colicin-A. Nature 354, 408-410.

van Gelder, P., de Cock, H. and Tommassen, J. (1994) Detergent-induced folding of the outer-membrane protein PhoE, a pore protein induced by phosphate limitation. Eur J Biochem 226, 783-787.

van Wijk, K. J., Knott, T. G. and Robinson, C. (1995) Evidence for SecA- and Delta pH-independent insertion of D1 into thylakoids. FEBS Lett 368, 263-266.

von Heijne, G. (1996) The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology. EMBO J 5, 3021-3027.

von Heijne, G. (2001) Control of topology and mode of assembly of a polytopic membrane protein by positively charged residues. Nature 341, 456-458.

von Heijne, G. (1992) Membrane Protein Structure Prediction - Hydrophobicity Analysis and the Positive-Inside Rule. J Mol Biol 225, 487-494.

von Heijne, G. (1994a) Membrane proteins: From sequence to structure. Annu Rev Biophys Biomol Struct 23, 167-192.

von Heijne, G. (1994b) Sec-independent protein insertion into the inner E. coli membrane: A phenomenon in search of an explanation. FEBS Lett 346, 69-72.

von Heijne, G. (1994c) Signal peptidases, R.G. Landes Press, Austin, TX.

von Heijne, G. (1996) Membrane protein assembly, R.G. Landes Press, Austin, TX.

von Heijne, G. and Gavel, Y. (1998) Topogenic signals in integral membrane proteins. Eur J Biochem 174, 671-678.

von Heijne, G. and Manoil, C. (1990) Membrane Proteins - From Sequence to Structure. Protein Eng 4, 109-112.

Voss, J., Hubbell, W. L. and Kaback, H. R. (1995) Distance determination in proteins using designed metal ion binding sites and site-directed spin labeling: Application to the lactose permease of Escherichia coli. Proc Natl Acad Sci USA 92, 12300-12303.

Wallin, E. and von Heijne, G. (1995) Properties of N-terminal tails in G-protein coupled receptors - A statistical study. Protein Engineer 8, 693-699.

Weiss, M. S., Abele, U., Weckesser, J., Welte, W., Schiltz, E. and Schulz, G. E. (1991a) Molecular architecture and electrostatic properties of a bacterial porin. Science 254, 1627-1630.

Weiss, M. S., Kreusch, A., Schiltz, E., Nestel, U., Welte, W., Weckesser, J. and Schulz, G. E. (1991b) The structure of porin from Rhodobacter capsulata at 1.8� resolution. FEBS Lett. 280, 379-382.

Welte, W., Weiss, M. S., Nestel, U., Weckesser, J., Schiltz, E. and Schulz, G. E. (1991) Prediction of the General Structure of OmpF and PhoE from the Sequence and Structure of Porin from Rhodobacter-Capsulatus - Orientation of Porin in the Membrane. Biochim Biophys Acta 1080, 271-274.

Wen, J. A., Chen, X. and Bowie, J. U. (1996) Exploring the allowed sequence space of a membrane protein. Nature Struct Biology 3, 141-148.

Wessels, H. P. and Spiess, M. (1998) Insertion of a multispanning membrane protein occurs sequentially and requires only one signal sequence. Cell 55, 61-70.

Whitley, P., Grahn, E., Kutay, U., Rapoport, T. and von Heijne, G. (1996) A 12 residues long poly-leucine tail is sufficient to anchor syntaptobrevin to the ER membrane. J Biol Chem 271, 7583-7586.

Whitley, P., Nilsson, I. and von Heijne, G. (1994a) De novo design of integral membrane proteins. Nature Struct Biol 1, 858-862.

Whitley, P., Nilsson, L. and von Heijne, G. (1993) 3-Dimensional model for the membrane domain of Escherichia coli leader peptidase based on disulfide mapping. Biochemistry 32, 8534-8539.

Whitley, P., Zander, T., Ehrmann, M., Haardt, M., Bremer, E. and von Heijne, G. (1994b) Sec-independent translocation of a 100-residues long periplasmic N-terminal tail in the E. coli inner membrane proteins ProW. EMBO J 13, 4653-4661.

Wolfe, P. B., Rice, M. and Wickner, W. (1995) Effects of two sec genes on protein assembly into the plasma membrane of Escherichia coli. J Biol Chem 260, 1836-1841.

Wu, J. H., Perrin, D. M., Sigman, D. S. and Kaback, H. R. (1995) Helix packing of lactose permease in Escherichia coli studied by site-directed chemical cleavage. Proc Natl Acad Sci USA 92, 9186-9190.

Yeates, T. O., Komiya, H., Rees, D. C., Allen, J. P. and Feher, G. (1997) Structure of the reaction center from Rhodobacter sphaeroides: Membrane-protein interactions. Proc Natl Acad Sci USA 84, 6438-6442.

Zhang, J. T., Lee, C. H., Duthie, M. and Ling, V. (1995) Topological determinants of internal transmembrane segments in P-glycoprotein sequences. J Biol Chem 270, 1742-1746.

Zhang, J. T. and Ling, V. (1991) Study of Membrane Orientation and Glycosylated Extracellular Loops of Mouse P-Glycoprotein by In vitro Translation. J Biol Chem 266, 18224-18232.