ENTRY TVHUF6 #type complete TITLE protein kinase raf-1 (EC 2.7.1.-) - human ALTERNATE_NAMES kinase-related transforming protein raf-1; raf-1 proto-oncogene protein-serine/threonine kinase CONTAINS protein kinase (EC 2.7.1.37) ORGANISM #formal_name Homo sapiens #common_name man DATE 04-Dec-1986 #sequence_revision 04-Dec-1986 #text_change 11-Jun-1999 ACCESSIONS A00637; I57580 REFERENCE A00637 #authors Bonner, T.I.; Oppermann, H.; Seeburg, P.; Kerby, S.B.; Gunnell, M.A.; Young, A.C.; Rapp, U.R. #journal Nucleic Acids Res. (1986) 14:1009-1015 #title The complete coding sequence of the human raf oncogene and the corresponding structure of the c-raf-1 gene. #cross-references MUID:86120351 #accession A00637 ##molecule_type mRNA ##residues 1-648 ##label BON1 ##cross-references GB:X03484; NID:g35841; PIDN:CAA27204.1; PID:g35842 REFERENCE I57580 #authors Bonner, T.I.; Kerby, S.B.; Sutrave, P.; Gunnell, M.A.; Mark, G.; Rapp, U.R. #journal Mol. Cell. Biol. (1985) 5:1400-1407 #title Structure and biological activity of human homologs of the raf/mil oncogene. #cross-references MUID:85295973 #accession I57580 ##status translated from GB/EMBL/DDBJ ##molecule_type DNA ##residues 228-239,'L',241-541,'I',543-648 ##label BON2 ##cross-references GB:L00212; NID:g190837; PIDN:AAA60247.1; PID:g496091 REFERENCE A43089 #authors Morrison, D.K.; Heidecker, G.; Rapp, U.R.; Copeland, T.D. #journal J. Biol. Chem. (1993) 268:17309-17316 #title Identification of the major phosphorylation sites of the Raf-1 kinase. #cross-references MUID:93352516 #contents annotation; phosphorylation sites #note expression is ubiquitous in mammalian tissues that have been studied COMMENT After phosphorylation and activation by protein kinase C and other kinases, this kinase is responsible for activating MAP kinase kinase (see PIR:A45100 and PIR:A46723). GENETICS #gene GDB:RAF1 ##cross-references GDB:119546; OMIM:164760 #map_position 3p25-3p25 #introns 278/3; 288/1; 330/3; 370/1; 398/2; 457/2; 473/1; 512/3; 556/3; 601/3 #note the list of introns is incomplete FUNCTION #description catalyzes the formation of specific peptidyl-threonine-phosphate and peptidyl-serine-phosphate residues in MAP kinase kinase using ATP #pathway MAP kinase cascade CLASSIFICATION #superfamily protein kinase A-raf; protein kinase C zinc-binding repeat homology; protein kinase homology KEYWORDS ATP; autophosphorylation; phosphoprotein; phosphotransferase; proto-oncogene; serine/ threonine-specific protein kinase; signal transduction; transforming protein; zinc FEATURE 139-184 #domain protein kinase C zinc-binding repeat homology #label KZN\ 347-613 #domain protein kinase homology #label KIN\ 355-363 #region protein kinase ATP-binding motif\ 43,621 #binding_site phosphate (Ser) (covalent) #status experimental\ 139,165,168,184 #binding_site zinc (His, Cys, Cys, Cys) #status predicted\ 152,155,173,176 #binding_site zinc (Cys, Cys, His, Cys) #status predicted\ 259 #binding_site phosphate (Ser) (covalent) (by protein kinase C) #status experimental\ 268 #binding_site phosphate (Thr) (covalent) (by autophosphorylation) #status experimental\ 375 #active_site Lys #status predicted\ 499 #binding_site phosphate (Ser) (covalent) (by protein kinase C) #status predicted SUMMARY #length 648 #molecular_weight 73051 SEQUENCE 5 10 15 20 25 30 1 M E H I Q G A W K T I S N G F G F K D A V F D G S S C I S P 31 T I V Q Q F G Y Q R R A S D D G K L T D P S K T S N T I R V 61 F L P N K Q R T V V N V R N G M S L H D C L M K A L K V R G 91 L Q P E C C A V F R L L H E H K G K K A R L D W N T D A A S 121 L I G E E L Q V D F L D H V P L T T H N F A R K T F L K L A 151 F C D I C Q K F L L N G F R C Q T C G Y K F H E H C S T K V 181 P T M C V D W S N I R Q L L L F P N S T I G D S G V P A L P 211 S L T M R R M R E S V S R M P V S S Q H R Y S T P H A F T F 241 N T S S P S S E G S L S Q R Q R S T S T P N V H M V S T T L 271 P V D S R M I E D A I R S H S E S A S P S A L S S S P N N L 301 S P T G W S Q P K T P V P A Q R E R A P V S G T Q E K N K I 331 R P R G Q R D S S Y Y W E I E A S E V M L S T R I G S G S F 361 G T V Y K G K W H G D V A V K I L K V V D P T P E Q F Q A F 391 R N E V A V L R K T R H V N I L L F M G Y M T K D N L A I V 421 T Q W C E G S S L Y K H L H V Q E T K F Q M F Q L I D I A R 451 Q T A Q G M D Y L H A K N I I H R D M K S N N I F L H E G L 481 T V K I G D F G L A T V K S R W S G S Q Q V E Q P T G S V L 511 W M A P E V I R M Q D N N P F S F Q S D V Y S Y G I V L Y E 541 L M T G E L P Y S H I N N R D Q I I F M V G R G Y A S P D L 571 S K L Y K N C P K A M K R L V A D C V K K V K E E R P L F P 601 Q I L S S I E L L Q H S L P K I N R S A S E P S L H R A A H 631 T E D I N A C T L T T S P R L P V F