Verification of protein structures

Protein structures might contain errors independently if they have been created from experimental information or from modeling. Therefore it is necessary to have some possibility to check for errors in a given model independent on how this model was created. The methods used to predict the structure of a protein can be divided into two groups, depending on if they are use physico chemical information or statistical information.

Methods using physicochemical information

Procheck

Procheck is a program that checks the geometry of a protein structure. The following checks are made:

  1. Covalent geometry
  2. Planarity
  3. Dihedral angles
  4. Chirality
  5. Non-bonded interactions
  6. Main-chain hydrogen bonds
  7. Disulphide bonds
  8. Stereochemical parameters
  9. Parameter comparisons
  10. Residue-by-residue analysis

WHAT Check

WHAT IF is a versatile protein structure analysis program that can be used for structure verification, energy calculations, molecular graphics, etc. It can be used to create automatic servers for protein verification as in the what check server

Methods using statistical analysis

Errat and Verify-3D

Errat and Verify-3D are bases on the statistical distribution of atoms and residues respectively.


Arne Elofsson
Last modified: Fri Oct 26 17:26:06 CEST 2001
Arne Elofsson
Stockholm Bioinformatics Center,
Department of Biochemistry,
Arrheniuslaboratoriet
Stockholms Universitet
10691 Stockholm, Sweden
Tel: +46-(0)8/161553
Fax: +46-(0)8/158057
Hem: +46-(0)8/6413158
Email: arne@sbc.su.se
WWW: /~arne/